Research peptide
KPV research peptide, 10 mg, lyophilized, over 99% HPLC purity
Synagenics KPV is a synthetic tripeptide supplied as a lyophilized research material for qualified laboratories. The certificate of analysis for lot KPV-10-0726 reports 99.94% HPLC purity with identity confirmed by LC-MS, and the full report is public.
KPV product data
Identifying details researchers confirm before ordering.
| Compound | KPV (Lys-Pro-Val) |
|---|---|
| Peptide sequence | Lys-Pro-Val, three amino acids |
| Molecular formula | C16H30N4O4 |
| Molecular weight | about 342.4 g/mol |
| CAS number | 67727-97-3 |
| Supplied amount | 10 mg per vial |
| Form | Lyophilized powder |
| Purity (HPLC) | Over 99%; 99.94% on lot KPV-10-0726 |
| Identity (LC-MS) | Confirmed on lot KPV-10-0726 |
| COA reported | July 4, 2026 |
| Grade | Research use only (RUO) |
About Synagenics KPV
KPV is a three-residue peptide made of lysine, proline and valine. Its sequence matches the C-terminal end of alpha-melanocyte-stimulating hormone (alpha-MSH), positions 11 to 13, which is why it is described in the literature as an alpha-MSH fragment.
In the laboratory, KPV serves as a small, defined peptide for cell culture work and for analytical method development. Because it is so short, it is also a useful test case for chromatography and mass spectrometry of low-molecular-weight peptides. For the chemistry, model systems and experimental design guidance, read our expert overview of peptide science.
What comes with every order
Documentation is part of the product.
Lot-tracked vial
The lot number is printed on the vial, so it matches the certificate in the public library.
Published certificate
HPLC chromatogram and purity, LC-MS identity and the report date, open to read before you order.
Research-use labeling
Clear RUO labeling on every vial, with no claims about people or animals.
Check the data before you order
You can verify our KPV without an account.
Open the COA library
Go to synagenics.com/coas/ and find the KPV 10 mg report.
Read the chromatogram
Look for one dominant peak on a flat baseline. The reported HPLC purity is 99.94%.
Check the identity
LC-MS should confirm the intact tripeptide at about 342.4 g/mol.
Match the lot
Confirm the lot number on your vial matches KPV-10-0726 or the lot named on the report you are shown.
Laboratory research applications
KPV appears in the literature across several in vitro and biochemical settings. These describe model systems, not any use beyond the bench.
- Cell culture models. Immortalized cell lines and primary cultures, with matched vehicle controls.
- Signaling readouts. Transcript and protein endpoints by RT-qPCR, ELISA or western blotting, chosen before the experiment starts.
- Peptide stability assays. LC-MS or HPLC tracking of the intact tripeptide in buffer or serum-containing medium.
- Uptake and transport studies. Labeled or mass-spectrometry-quantified peptide in cell monolayer assays.
- Analytical method development. A short, polar reference peptide for testing retention, resolution and mass accuracy.
General design guidance and controls are in the Cellular Research Hub.
Why short peptides need careful analysis
A tripeptide such as KPV is small and polar, and it carries a basic lysine side chain. On a reversed-phase column it elutes early, sometimes close to the solvent front, so a poorly tuned method can hide impurities under the void signal or inflate the apparent purity.
That is why the method matters as much as the number. When you review a certificate, look at where the main peak sits, how clean the baseline is around it, and whether the mass spectrum shows the expected ion near 342.4 g/mol. Chromatographic purity alone cannot tell you that the peak is the right molecule, and a mass spectrum alone cannot tell you how much of the sample it represents. You want both.
Plan your purchase for the whole study
- Estimate total need. Calculate how much peptide the full study will consume, including repeats, and allow a margin.
- Prefer one lot. Where practical, source the study from a single lot and record the lot number with every dataset.
- Account for net peptide content. Powder mass includes counter-ion and bound water, so concentrations calculated from weight alone can be too high. This matters more for a small peptide, where the counter-ion is a larger share of the mass.
- Use matched controls. A scrambled tripeptide and a vehicle-only arm help separate sequence effects from handling effects.
- State the medium. Serum contains peptidases, so record the serum percentage and consider a serum-free condition.
How to compare KPV suppliers
KPV product pages look alike. The documentation is what differs.
| Lot-specific certificate | A report that names the exact lot you will receive, not a generic sheet. |
|---|---|
| Full chromatogram | The trace itself, not only a percentage. |
| Identity by mass spectrometry | Observed mass near 342.4 g/mol for the intact tripeptide. |
| Independent testing | Third-party data rather than a self-issued report. |
| Stated identifiers | Sequence, molecular weight and CAS number that match the compound you intend to use. |
| Research-use language | A supplier that implies use in people is not operating as a research supplier. |
Responsible purchasing for research
Synagenics supplies KPV to qualified laboratories and researchers for laboratory use only. Buyers are responsible for using the material lawfully and in line with their institution’s procurement, safety and ethics policies. The product is not a drug, supplement or cosmetic, and nothing on this page is a claim about use in people or animals.
Questions? Email support@synagenics.com. To learn how we test every lot, read Setting the Standard at Synagenics and From Our Lab.
Key takeaways for KPV buyers
- Read the chromatogram and the mass spectrum together: purity and identity answer different questions.
- Match the lot on your vial to the certificate before you use it.
- Calculate concentrations from net peptide content where it is available.
- Source a long study from one lot where practical.
- Use KPV strictly for laboratory research, within your institution’s policies.
KPV in context: what small peptide fragments are used to study
Researchers have long used short peptide fragments to ask which part of a larger signaling molecule carries a given behavior in a model system. Alpha-MSH is a thirteen-residue peptide, and KPV is its three-residue C-terminal piece. Comparing the fragment with the parent peptide, and with scrambled controls, separates the effect of a short sequence from the effect of the whole molecule.
Size also changes the practical questions. A tripeptide crosses cell monolayers differently from a long peptide, is cleared differently by peptidases and is easier to quantify by mass spectrometry. Good experiments therefore measure the intact peptide at the start and end of an incubation and report the stability they observed under their own conditions, rather than assuming the dose in the well stayed constant.
Designing a clean in vitro experiment with KPV
- Pick the right controls. Use a vehicle control, a scrambled tripeptide and, where relevant, the parent peptide for comparison.
- Measure the intact peptide. Use LC-MS or HPLC to confirm how much KPV remains at the end of the incubation.
- Standardize the cells. Hold passage number, plating density and culture conditions constant across arms.
- Define the endpoint in advance. Decide the readout, the time points and the statistical test before you start.
- Replicate independently. Independent biological replicates started on separate days matter more than many wells in one run.
- Record the lot. Keep the lot number with every dataset so the work can be repeated.
Understanding the numbers on the KPV certificate
The certificate for lot KPV-10-0726 reports a purity of 99.94% by HPLC. That figure means the main peak accounts for 99.94 percent of the integrated area in the chromatogram. It does not say how much of the powder is peptide, which is why net peptide content is a separate question when you calculate a stock concentration.
The identity result comes from LC-MS, which separates the sample by chromatography and then measures the mass of what elutes. For KPV the expected mass is about 342.4 g/mol, and the report confirms that the intact peptide is present. Open the report in the COA library and compare the chromatogram and the mass spectrum yourself. New lots are tested the same way, and purity is expected to stay over 99%.
Common questions researchers ask before using KPV
- How stable is the peptide in my medium?. Measure it. Serum peptidases vary by lot, so confirm the intact peptide by LC-MS at the end of the incubation.
- Do I need a serum-free control?. It is a useful comparison, because it separates peptide stability from peptide activity.
- Which mass should I expect?. About 342.4 g/mol for the intact tripeptide.
- What should I record?. The lot number, the net peptide content used for concentration, the medium, the serum percentage and the incubation time.
Our Research Peptide Quality, Testing and COA Guide explains how to read HPLC and mass spectrometry data in more depth.
Buying KPV for research: FAQ
What is Synagenics KPV?
It is the synthetic tripeptide Lys-Pro-Val, supplied as a 10 mg lyophilized research material.
Is KPV sold for human or animal use?
No. It is sold strictly for qualified laboratory research. It is not a drug, supplement or cosmetic, and nothing on this page is a claim about use in people or animals.
What purity does the KPV certificate report?
The certificate for lot KPV-10-0726 reports 99.94% purity by HPLC, with identity confirmed by LC-MS, reported July 4, 2026. New lots are tested the same way and are expected to report over 99%.
Where can I read the certificate?
In the public Certificate of Analysis library at synagenics.com/coas/, with no account required.
Why is a short peptide harder to analyze?
A tripeptide is small and polar, so it elutes early on a reversed-phase column and can sit close to the solvent front. A method that resolves it from void-volume signals and a mass spectrum that confirms the intact mass give you more confidence than a percentage alone.
Who do I contact with questions?
Email support@synagenics.com with the compound name and your lot number if you have one.
Related research
Continue exploring the Synagenics research library.
Order research-grade KPV
Independently tested, with the certificate published for you to read first.
For qualified laboratory research use only. Not for human or animal use. These statements have not been evaluated by the US Food and Drug Administration.